Coding

Part:BBa_K4899005:Design

Designed by: Morten Pedersen Hauger   Group: iGEM23_UiOslo-Norway   (2023-10-11)


strep tagged dcas12f (dcasMINI)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1354
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1354
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1354
    Illegal BglII site found at 287
    Illegal BglII site found at 296
    Illegal BglII site found at 365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1354
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ensure that dimer formation was not inhibited by addition of tags


Source

modifed version of BBa_K4899004, based on lieterture

References